Contact an Account ManagerSeparation of PAHs on a 2mm ID column
Summary
Type: HPLC
Application #: 101700
Catalog#: 720117.20 - MN HPLC Column, Nucleosil C18 PAH, 100A, 5um, 2x250mm
Detection: fluorescence, wavelength program (ex / em):
1. 275/350nm,
2. 260/390nm,
3. 260/420nm,
4. 290/430nm,
5. 300/500nm
Conditions: eluent: A) methanol - water (55:45), B) acetonitrile - tetrahydrofuran (93:7),
gradient: 3min isocratic 120% B, then linear gradient 10 - 60% B in 27min, 60 to 80% B in 20min, 80 to 95% B in 5min, finally 15min 95% B isocratic, equilibrate column 10min with 10% B for next injection,
flow rate 0.2ml/min,
temperature 28°C
Authors: Winkeler H-D
Source: GIT Spezial Chromatographie 2/92 80 - 85
Details
Legend: a) PAH standards according to EPA + benzo[e]pyrene and binaphthyl as internal
standard,
b) PAHs from a soil sample,
c) PAHs from a wall-to-wall carpeting from the sleeping room of a smoker,
sample